Activity of the mouse Notch ligand DLL1 is sensitive to C-terminal tagging in vivo

OBJECTIVE: The mammalian Notch ligand DLL1 has essential functions during development. To visualise DLL1 in tissues, for sorting and enrichment of DLL1-expressing cells, and to efficiently purify DLL1 protein complexes we tagged DLL1 in mice with AcGFPHA or Strep/FLAG. RESULTS: We generated construc...

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Detalles Bibliográficos
Autores principales: Schuster-Gossler, Karin, Boldt, Karsten, Bornhorst, Dorothee, Delany-Heiken, Patricia, Ueffing, Marius, Gossler, Achim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477538/
https://www.ncbi.nlm.nih.gov/pubmed/34583743
http://dx.doi.org/10.1186/s13104-021-05785-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477538/
https://www.ncbi.nlm.nih.gov/pubmed/34583743
http://dx.doi.org/10.1186/s13104-021-05785-4

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