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New Red-Emitting Chloride-Sensitive Fluorescent Protein with Biological Uses

[Image: see text] A new chloride-sensitive red fluorescent protein derived from Entacmaea quadricolor is described. We found that mBeRFP exhibited moderate sensitivity to chloride and, via site-directed mutagenesis (S94V and R205Y), we increased the chloride affinity by more than an order of magnitu...

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Detalles Bibliográficos
Autores principales: Salto, Rafael, Giron, Maria D., Puente-Muñoz, Virginia, Vilchez, Jose D., Espinar-Barranco, Laura, Valverde-Pozo, Javier, Arosio, Daniele, Paredes, Jose M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8478333/
https://www.ncbi.nlm.nih.gov/pubmed/34148347
http://dx.doi.org/10.1021/acssensors.1c00094