Enhanced Cas12a multi-gene regulation using a CRISPR array separator

The type V-A Cas12a protein can process its CRISPR array, a feature useful for multiplexed gene editing and regulation. However, CRISPR arrays often exhibit unpredictable performance due to interference between multiple guide RNA (gRNAs). Here, we report that Cas12a array performance is hypersensiti...

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Detalles Bibliográficos
Autores principales: Magnusson, Jens P, Rios, Antonio Ray, Wu, Lingling, Qi, Lei S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Chromosomes and Gene Expression
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8478413/
https://www.ncbi.nlm.nih.gov/pubmed/34499031
http://dx.doi.org/10.7554/eLife.66406

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8478413/
https://www.ncbi.nlm.nih.gov/pubmed/34499031
http://dx.doi.org/10.7554/eLife.66406

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