Replacing the SpCas9 HNH domain by deaminases generates compact base editors with an alternative targeting scope

Base editors are RNA-guided deaminases that enable site-specific nucleotide transitions. The targeting scope of these Cas-deaminase fusion proteins critically depends on the availability of a protospacer adjacent motif (PAM) at the target locus and is limited to a window within the CRISPR-Cas R-loop...

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Detalles Bibliográficos
Autores principales: Villiger, Lukas, Schmidheini, Lukas, Mathis, Nicolas, Rothgangl, Tanja, Marquart, Kim, Schwank, Gerald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2021
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8479293/
https://www.ncbi.nlm.nih.gov/pubmed/34631280
http://dx.doi.org/10.1016/j.omtn.2021.08.025

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8479293/
https://www.ncbi.nlm.nih.gov/pubmed/34631280
http://dx.doi.org/10.1016/j.omtn.2021.08.025

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