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In silico study of intrinsic dynamics of full-length apo-ACE2 and RBD-ACE2 complex
The key step for SARS-CoV-2 to infect human cells is the membrane fusion triggered by the binding of the viral extracellular Spike protein to the human extracellular receptor, the angiotensin-converting enzyme 2 (ACE2). Although the Cryo-electron microscopy (Cryo-EM) uncovered the static atomic deta...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Research Network of Computational and Structural Biotechnology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8479424/ https://www.ncbi.nlm.nih.gov/pubmed/34603638 http://dx.doi.org/10.1016/j.csbj.2021.09.032 |
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author | Liu, Qing Wang, Yuwei Leung, Elaine Lai-Han Yao, Xiaojun |
author_facet | Liu, Qing Wang, Yuwei Leung, Elaine Lai-Han Yao, Xiaojun |
author_sort | Liu, Qing |
collection | PubMed |
description | The key step for SARS-CoV-2 to infect human cells is the membrane fusion triggered by the binding of the viral extracellular Spike protein to the human extracellular receptor, the angiotensin-converting enzyme 2 (ACE2). Although the Cryo-electron microscopy (Cryo-EM) uncovered the static atomic details of ACE2 homodimers, there is still a lack of research on the kinetic and thermodynamic properties of these full-length structures. This information is helpful to understand and interpret the role of ACE2 in the cell entry of SARS-CoV-2. In order to obtain this information, we performed microsecond-scale conventional and accelerated molecular dynamics (MD) simulations of full-length all-atomic systems of the RBD-ACE2 complex, the normal and torsional conformations of the apo-ACE2 homodimer. The comparative analysis of these systems showed that there were differences in their allosteric signal pathways and motion trends. These results may be helpful to further explore the cell entry mechanism of SARS-CoV-2. Moreover, the binding free energy and hydrogen bond distribution analysis of RBD-ACE2 binding interface provided the binding motifs that may be critical to allosteric signal transmission and RBD binding. These multi-conformational binding motifs can be used as targets or templates for the inhibitor design of the cell entry of SARS-CoV-2. |
format | Online Article Text |
id | pubmed-8479424 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Research Network of Computational and Structural Biotechnology |
record_format | MEDLINE/PubMed |
spelling | pubmed-84794242021-09-29 In silico study of intrinsic dynamics of full-length apo-ACE2 and RBD-ACE2 complex Liu, Qing Wang, Yuwei Leung, Elaine Lai-Han Yao, Xiaojun Comput Struct Biotechnol J Research Article The key step for SARS-CoV-2 to infect human cells is the membrane fusion triggered by the binding of the viral extracellular Spike protein to the human extracellular receptor, the angiotensin-converting enzyme 2 (ACE2). Although the Cryo-electron microscopy (Cryo-EM) uncovered the static atomic details of ACE2 homodimers, there is still a lack of research on the kinetic and thermodynamic properties of these full-length structures. This information is helpful to understand and interpret the role of ACE2 in the cell entry of SARS-CoV-2. In order to obtain this information, we performed microsecond-scale conventional and accelerated molecular dynamics (MD) simulations of full-length all-atomic systems of the RBD-ACE2 complex, the normal and torsional conformations of the apo-ACE2 homodimer. The comparative analysis of these systems showed that there were differences in their allosteric signal pathways and motion trends. These results may be helpful to further explore the cell entry mechanism of SARS-CoV-2. Moreover, the binding free energy and hydrogen bond distribution analysis of RBD-ACE2 binding interface provided the binding motifs that may be critical to allosteric signal transmission and RBD binding. These multi-conformational binding motifs can be used as targets or templates for the inhibitor design of the cell entry of SARS-CoV-2. Research Network of Computational and Structural Biotechnology 2021-09-29 /pmc/articles/PMC8479424/ /pubmed/34603638 http://dx.doi.org/10.1016/j.csbj.2021.09.032 Text en © 2021 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Liu, Qing Wang, Yuwei Leung, Elaine Lai-Han Yao, Xiaojun In silico study of intrinsic dynamics of full-length apo-ACE2 and RBD-ACE2 complex |
title | In silico study of intrinsic dynamics of full-length apo-ACE2 and RBD-ACE2 complex |
title_full | In silico study of intrinsic dynamics of full-length apo-ACE2 and RBD-ACE2 complex |
title_fullStr | In silico study of intrinsic dynamics of full-length apo-ACE2 and RBD-ACE2 complex |
title_full_unstemmed | In silico study of intrinsic dynamics of full-length apo-ACE2 and RBD-ACE2 complex |
title_short | In silico study of intrinsic dynamics of full-length apo-ACE2 and RBD-ACE2 complex |
title_sort | in silico study of intrinsic dynamics of full-length apo-ace2 and rbd-ace2 complex |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8479424/ https://www.ncbi.nlm.nih.gov/pubmed/34603638 http://dx.doi.org/10.1016/j.csbj.2021.09.032 |
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