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FRC-QE: a robust and comparable 3D microscopy image quality metric for cleared organoids

SUMMARY: Here, we propose Fourier ring correlation-based quality estimation (FRC-QE) as a new metric for automated image quality estimation in 3D fluorescence microscopy acquisitions of cleared organoids that yields comparable measurements across experimental replicates, clearing protocols and works...

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Detalles Bibliográficos
Autores principales: Preusser, Friedrich, dos Santos, Natália, Contzen, Jörg, Stachelscheid, Harald, Costa, Érico Tosoni, Mergenthaler, Philipp, Preibisch, Stephan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8479654/
https://www.ncbi.nlm.nih.gov/pubmed/33693580
http://dx.doi.org/10.1093/bioinformatics/btab160