Protocol for preparation and measurement of intracellular and extracellular modified RNA using liquid chromatography-mass spectrometry

About 150 modifications have been identified in RNA species. Besides their regulatory roles in the intracellular gene expression, abundant modified RNA nucleosides are catabolized from RNA and released into extracellular fluids, which can impact extracellular signaling as ligands for receptors. Here...

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Detalles Bibliográficos
Autores principales: Ogawa, Akiko, Wei, Fan-Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482036/
https://www.ncbi.nlm.nih.gov/pubmed/34622220
http://dx.doi.org/10.1016/j.xpro.2021.100848
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author Ogawa, Akiko
Wei, Fan-Yan
author_facet Ogawa, Akiko
Wei, Fan-Yan
author_sort Ogawa, Akiko
collection PubMed
description About 150 modifications have been identified in RNA species. Besides their regulatory roles in the intracellular gene expression, abundant modified RNA nucleosides are catabolized from RNA and released into extracellular fluids, which can impact extracellular signaling as ligands for receptors. Here, we describe a protocol to prepare samples from biological specimens, including cultured cells, extracellular fluid, and tissues, to measure both intracellular and extracellular RNA modifications using mass spectrometry. For complete details on the use and execution of this protocol, please refer to Ogawa et al. (2021).
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spelling pubmed-84820362021-10-06 Protocol for preparation and measurement of intracellular and extracellular modified RNA using liquid chromatography-mass spectrometry Ogawa, Akiko Wei, Fan-Yan STAR Protoc Protocol About 150 modifications have been identified in RNA species. Besides their regulatory roles in the intracellular gene expression, abundant modified RNA nucleosides are catabolized from RNA and released into extracellular fluids, which can impact extracellular signaling as ligands for receptors. Here, we describe a protocol to prepare samples from biological specimens, including cultured cells, extracellular fluid, and tissues, to measure both intracellular and extracellular RNA modifications using mass spectrometry. For complete details on the use and execution of this protocol, please refer to Ogawa et al. (2021). Elsevier 2021-09-27 /pmc/articles/PMC8482036/ /pubmed/34622220 http://dx.doi.org/10.1016/j.xpro.2021.100848 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ogawa, Akiko
Wei, Fan-Yan
Protocol for preparation and measurement of intracellular and extracellular modified RNA using liquid chromatography-mass spectrometry
title Protocol for preparation and measurement of intracellular and extracellular modified RNA using liquid chromatography-mass spectrometry
title_full Protocol for preparation and measurement of intracellular and extracellular modified RNA using liquid chromatography-mass spectrometry
title_fullStr Protocol for preparation and measurement of intracellular and extracellular modified RNA using liquid chromatography-mass spectrometry
title_full_unstemmed Protocol for preparation and measurement of intracellular and extracellular modified RNA using liquid chromatography-mass spectrometry
title_short Protocol for preparation and measurement of intracellular and extracellular modified RNA using liquid chromatography-mass spectrometry
title_sort protocol for preparation and measurement of intracellular and extracellular modified rna using liquid chromatography-mass spectrometry
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482036/
https://www.ncbi.nlm.nih.gov/pubmed/34622220
http://dx.doi.org/10.1016/j.xpro.2021.100848
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