Scaling production of GFP1-10 detector protein in E. coli for secretion screening by split GFP assay

BACKGROUND: The split GFP assay is a well-known technology for activity-independent screening of target proteins. A superfolder GFP is split into two non-fluorescent parts, GFP11 which is fused to the target protein and GFP1-10. In the presence of both, GFP1-10 and the GFP11-tag are self-assembled a...

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Detalles Bibliográficos
Autores principales: Müller, Carolin, Igwe, Chika L., Wiechert, Wolfgang, Oldiges, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Technical Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482599/
https://www.ncbi.nlm.nih.gov/pubmed/34592997
http://dx.doi.org/10.1186/s12934-021-01672-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482599/
https://www.ncbi.nlm.nih.gov/pubmed/34592997
http://dx.doi.org/10.1186/s12934-021-01672-6

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