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Scaling production of GFP1-10 detector protein in E. coli for secretion screening by split GFP assay
BACKGROUND: The split GFP assay is a well-known technology for activity-independent screening of target proteins. A superfolder GFP is split into two non-fluorescent parts, GFP11 which is fused to the target protein and GFP1-10. In the presence of both, GFP1-10 and the GFP11-tag are self-assembled a...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482599/ https://www.ncbi.nlm.nih.gov/pubmed/34592997 http://dx.doi.org/10.1186/s12934-021-01672-6 |