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COL5A1 Variants Cause Aortic Dissection by Activating TGF‐β‐Signaling Pathway
BACKGROUND: Aortic dissection (AD) is one of the most life‐threatening cardiovascular diseases that exhibit high genetic heterogeneity. However, it is unclear whether variants within the COL5A1 gene can cause AD. Therefore, we intend to determine whether COL5A1 is a causative gene of AD. METHODS AND...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8483548/ https://www.ncbi.nlm.nih.gov/pubmed/34041919 http://dx.doi.org/10.1161/JAHA.120.019276 |
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author | Chen, Peng Yu, Bo Li, Zongzhe Chen, Yanghui Sun, Yang Wang, Dao Wen |
author_facet | Chen, Peng Yu, Bo Li, Zongzhe Chen, Yanghui Sun, Yang Wang, Dao Wen |
author_sort | Chen, Peng |
collection | PubMed |
description | BACKGROUND: Aortic dissection (AD) is one of the most life‐threatening cardiovascular diseases that exhibit high genetic heterogeneity. However, it is unclear whether variants within the COL5A1 gene can cause AD. Therefore, we intend to determine whether COL5A1 is a causative gene of AD. METHODS AND RESULTS: We performed targeted sequencing in 702 patients with unrelated sporadic AD and 163 matched healthy controls using a predesigned panel with 152 vessel matrix‐related genes. As a result, we identified that 11 variants in COL5A1 caused AD in 11 out of the 702 patients with AD. Furthermore, Col5a1 knockout (Col5a1(+/−) ) rats were generated through the CRISPR/Cas9 system. Although there was no spontaneous AD, electron microscopy revealed a fracture of elastic fibers and disarray of collagenous fibers in 6‐week‐old Col5a1(+/−) rats, but not in WT rats (93.3% versus 0.0%, P<0.001). Three‐week‐old rats were used to induce the AD phenotype with β‐aminopropionitrile monofumarate for 4 weeks followed by angiotensin II for 72 hours. The β‐aminopropionitrile monofumarate and angiotensin II‐treated rat model confirmed that Col5a1(+/−) rats had considerably higher AD incidence than WT rats. Subsequent mechanism analyses demonstrated that the transforming growth factor‐β‐signaling pathway was significantly activated in Col5a1(+/−) rats. CONCLUSIONS: Our findings, for the first time, revealed a relationship between variants in COL5A1 and AD via targeted sequencing in 1.57% patients with sporadic aortic dissection. The Col5a1 knockout rats exhibited AD after an intervention, indicating that COL5A1 is a causative gene of AD. Activation of the transforming growth factor‐β‐signaling pathway may be implicated in the pathogenesis of this kind of AD. |
format | Online Article Text |
id | pubmed-8483548 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84835482021-10-06 COL5A1 Variants Cause Aortic Dissection by Activating TGF‐β‐Signaling Pathway Chen, Peng Yu, Bo Li, Zongzhe Chen, Yanghui Sun, Yang Wang, Dao Wen J Am Heart Assoc Original Research BACKGROUND: Aortic dissection (AD) is one of the most life‐threatening cardiovascular diseases that exhibit high genetic heterogeneity. However, it is unclear whether variants within the COL5A1 gene can cause AD. Therefore, we intend to determine whether COL5A1 is a causative gene of AD. METHODS AND RESULTS: We performed targeted sequencing in 702 patients with unrelated sporadic AD and 163 matched healthy controls using a predesigned panel with 152 vessel matrix‐related genes. As a result, we identified that 11 variants in COL5A1 caused AD in 11 out of the 702 patients with AD. Furthermore, Col5a1 knockout (Col5a1(+/−) ) rats were generated through the CRISPR/Cas9 system. Although there was no spontaneous AD, electron microscopy revealed a fracture of elastic fibers and disarray of collagenous fibers in 6‐week‐old Col5a1(+/−) rats, but not in WT rats (93.3% versus 0.0%, P<0.001). Three‐week‐old rats were used to induce the AD phenotype with β‐aminopropionitrile monofumarate for 4 weeks followed by angiotensin II for 72 hours. The β‐aminopropionitrile monofumarate and angiotensin II‐treated rat model confirmed that Col5a1(+/−) rats had considerably higher AD incidence than WT rats. Subsequent mechanism analyses demonstrated that the transforming growth factor‐β‐signaling pathway was significantly activated in Col5a1(+/−) rats. CONCLUSIONS: Our findings, for the first time, revealed a relationship between variants in COL5A1 and AD via targeted sequencing in 1.57% patients with sporadic aortic dissection. The Col5a1 knockout rats exhibited AD after an intervention, indicating that COL5A1 is a causative gene of AD. Activation of the transforming growth factor‐β‐signaling pathway may be implicated in the pathogenesis of this kind of AD. John Wiley and Sons Inc. 2021-05-27 /pmc/articles/PMC8483548/ /pubmed/34041919 http://dx.doi.org/10.1161/JAHA.120.019276 Text en © 2021 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Research Chen, Peng Yu, Bo Li, Zongzhe Chen, Yanghui Sun, Yang Wang, Dao Wen COL5A1 Variants Cause Aortic Dissection by Activating TGF‐β‐Signaling Pathway |
title | COL5A1 Variants Cause Aortic Dissection by Activating TGF‐β‐Signaling Pathway |
title_full | COL5A1 Variants Cause Aortic Dissection by Activating TGF‐β‐Signaling Pathway |
title_fullStr | COL5A1 Variants Cause Aortic Dissection by Activating TGF‐β‐Signaling Pathway |
title_full_unstemmed | COL5A1 Variants Cause Aortic Dissection by Activating TGF‐β‐Signaling Pathway |
title_short | COL5A1 Variants Cause Aortic Dissection by Activating TGF‐β‐Signaling Pathway |
title_sort | col5a1 variants cause aortic dissection by activating tgf‐β‐signaling pathway |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8483548/ https://www.ncbi.nlm.nih.gov/pubmed/34041919 http://dx.doi.org/10.1161/JAHA.120.019276 |
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