Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair

CRISPR–Cas proteins are RNA-guided nucleases used to introduce double-stranded breaks (DSBs) at targeted genomic loci. DSBs are repaired by endogenous cellular pathways such as non-homologous end joining (NHEJ) and homology-directed repair (HDR). Providing an exogenous DNA template during repair all...

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Detalles Bibliográficos
Autores principales: Schubert, Mollie S., Thommandru, Bernice, Woodley, Jessica, Turk, Rolf, Yan, Shuqi, Kurgan, Gavin, McNeill, Matthew S., Rettig, Garrett R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484621/
https://www.ncbi.nlm.nih.gov/pubmed/34593942
http://dx.doi.org/10.1038/s41598-021-98965-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484621/
https://www.ncbi.nlm.nih.gov/pubmed/34593942
http://dx.doi.org/10.1038/s41598-021-98965-y

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