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Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair
CRISPR–Cas proteins are RNA-guided nucleases used to introduce double-stranded breaks (DSBs) at targeted genomic loci. DSBs are repaired by endogenous cellular pathways such as non-homologous end joining (NHEJ) and homology-directed repair (HDR). Providing an exogenous DNA template during repair all...
Autores principales: | Schubert, Mollie S., Thommandru, Bernice, Woodley, Jessica, Turk, Rolf, Yan, Shuqi, Kurgan, Gavin, McNeill, Matthew S., Rettig, Garrett R. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484621/ https://www.ncbi.nlm.nih.gov/pubmed/34593942 http://dx.doi.org/10.1038/s41598-021-98965-y |
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