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Identification of Oleanolic Acid as Allosteric Agonist of Integrin α(M) by Combination of In Silico Modeling and In Vitro Analysis
Oleanolic acid is a widely distributed natural product, which possesses promising antitumor, antiviral, antihyperlipidemic, and anti-inflammatory activities. A heterodimeric complex formed by integrin α(M) (CD11b) and integrin β(2) (CD18) is highly expressed on monocytes and macrophages. In the curr...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484648/ https://www.ncbi.nlm.nih.gov/pubmed/34603018 http://dx.doi.org/10.3389/fphar.2021.702529 |
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author | Jin, Lu Han, Xiaoyu Zhang, Xinlei Zhao, Zhimin Ulrich, Judith Syrovets, Tatiana Simmet, Thomas |
author_facet | Jin, Lu Han, Xiaoyu Zhang, Xinlei Zhao, Zhimin Ulrich, Judith Syrovets, Tatiana Simmet, Thomas |
author_sort | Jin, Lu |
collection | PubMed |
description | Oleanolic acid is a widely distributed natural product, which possesses promising antitumor, antiviral, antihyperlipidemic, and anti-inflammatory activities. A heterodimeric complex formed by integrin α(M) (CD11b) and integrin β(2) (CD18) is highly expressed on monocytes and macrophages. In the current study, we demonstrate that the I domain of α(M) (α(M)-I domain) might present a potential cellular target for oleanolic acid. In vitro data show that oleanolic acid induces clustering of α(M) on macrophages and reduces their non-directional migration. In accordance with experimental data, molecular docking revealed that oleanolic acid binds to the α(M)-I domain in its extended-open form, the dominant conformation found in α(M) clusters. Molecular dynamics simulation revealed that oleanolic acid can increase the flexibility of the α7 helix and promote its movement away from the N-terminus, indicating that oleanolic acid may facilitate the conversion of the α(M)-I domain from the extended-closed to the extended-open conformation. As demonstrated by metadynamics simulation, oleanolic acid can destabilize the local minimum of the α(M)-I domain in the open conformation partially through disturbance of the interactions between α1 and α7 helices. In summary, we demonstrate that oleanolic acid might function as an allosteric agonist inducing clustering of α(M) on macrophages by shifting the balance from the closed to the extended-open conformation. The molecular target identified in this study might hold potential for a purposeful use of oleanolic acid to modulate chronic inflammatory responses. |
format | Online Article Text |
id | pubmed-8484648 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84846482021-10-02 Identification of Oleanolic Acid as Allosteric Agonist of Integrin α(M) by Combination of In Silico Modeling and In Vitro Analysis Jin, Lu Han, Xiaoyu Zhang, Xinlei Zhao, Zhimin Ulrich, Judith Syrovets, Tatiana Simmet, Thomas Front Pharmacol Pharmacology Oleanolic acid is a widely distributed natural product, which possesses promising antitumor, antiviral, antihyperlipidemic, and anti-inflammatory activities. A heterodimeric complex formed by integrin α(M) (CD11b) and integrin β(2) (CD18) is highly expressed on monocytes and macrophages. In the current study, we demonstrate that the I domain of α(M) (α(M)-I domain) might present a potential cellular target for oleanolic acid. In vitro data show that oleanolic acid induces clustering of α(M) on macrophages and reduces their non-directional migration. In accordance with experimental data, molecular docking revealed that oleanolic acid binds to the α(M)-I domain in its extended-open form, the dominant conformation found in α(M) clusters. Molecular dynamics simulation revealed that oleanolic acid can increase the flexibility of the α7 helix and promote its movement away from the N-terminus, indicating that oleanolic acid may facilitate the conversion of the α(M)-I domain from the extended-closed to the extended-open conformation. As demonstrated by metadynamics simulation, oleanolic acid can destabilize the local minimum of the α(M)-I domain in the open conformation partially through disturbance of the interactions between α1 and α7 helices. In summary, we demonstrate that oleanolic acid might function as an allosteric agonist inducing clustering of α(M) on macrophages by shifting the balance from the closed to the extended-open conformation. The molecular target identified in this study might hold potential for a purposeful use of oleanolic acid to modulate chronic inflammatory responses. Frontiers Media S.A. 2021-09-17 /pmc/articles/PMC8484648/ /pubmed/34603018 http://dx.doi.org/10.3389/fphar.2021.702529 Text en Copyright © 2021 Jin, Han, Zhang, Zhao, Ulrich, Syrovets and Simmet. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Jin, Lu Han, Xiaoyu Zhang, Xinlei Zhao, Zhimin Ulrich, Judith Syrovets, Tatiana Simmet, Thomas Identification of Oleanolic Acid as Allosteric Agonist of Integrin α(M) by Combination of In Silico Modeling and In Vitro Analysis |
title | Identification of Oleanolic Acid as Allosteric Agonist of Integrin α(M) by Combination of In Silico Modeling and In Vitro Analysis |
title_full | Identification of Oleanolic Acid as Allosteric Agonist of Integrin α(M) by Combination of In Silico Modeling and In Vitro Analysis |
title_fullStr | Identification of Oleanolic Acid as Allosteric Agonist of Integrin α(M) by Combination of In Silico Modeling and In Vitro Analysis |
title_full_unstemmed | Identification of Oleanolic Acid as Allosteric Agonist of Integrin α(M) by Combination of In Silico Modeling and In Vitro Analysis |
title_short | Identification of Oleanolic Acid as Allosteric Agonist of Integrin α(M) by Combination of In Silico Modeling and In Vitro Analysis |
title_sort | identification of oleanolic acid as allosteric agonist of integrin α(m) by combination of in silico modeling and in vitro analysis |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484648/ https://www.ncbi.nlm.nih.gov/pubmed/34603018 http://dx.doi.org/10.3389/fphar.2021.702529 |
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