The Off-Targets of Clustered Regularly Interspaced Short Palindromic Repeats Gene Editing

The repurposing of the CRISPR/Cas bacterial defense system against bacteriophages as simple and flexible molecular tools has revolutionized the field of gene editing. These tools are now widely used in basic research and clinical trials involving human somatic cells. However, a global moratorium on...

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Autores principales: Vicente, Manuel M., Chaves-Ferreira, Miguel, Jorge, João M. P., Proença, João T., Barreto, Vasco M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Cell and Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484971/
https://www.ncbi.nlm.nih.gov/pubmed/34604217
http://dx.doi.org/10.3389/fcell.2021.718466
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author Vicente, Manuel M.
Chaves-Ferreira, Miguel
Jorge, João M. P.
Proença, João T.
Barreto, Vasco M.
author_facet Vicente, Manuel M.
Chaves-Ferreira, Miguel
Jorge, João M. P.
Proença, João T.
Barreto, Vasco M.
author_sort Vicente, Manuel M.
collection PubMed
description The repurposing of the CRISPR/Cas bacterial defense system against bacteriophages as simple and flexible molecular tools has revolutionized the field of gene editing. These tools are now widely used in basic research and clinical trials involving human somatic cells. However, a global moratorium on all clinical uses of human germline editing has been proposed because the technology still lacks the required efficacy and safety. Here we focus on the approaches developed since 2013 to decrease the frequency of unwanted mutations (the off-targets) during CRISPR-based gene editing.
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spelling pubmed-84849712021-10-02 The Off-Targets of Clustered Regularly Interspaced Short Palindromic Repeats Gene Editing Vicente, Manuel M. Chaves-Ferreira, Miguel Jorge, João M. P. Proença, João T. Barreto, Vasco M. Front Cell Dev Biol Cell and Developmental Biology The repurposing of the CRISPR/Cas bacterial defense system against bacteriophages as simple and flexible molecular tools has revolutionized the field of gene editing. These tools are now widely used in basic research and clinical trials involving human somatic cells. However, a global moratorium on all clinical uses of human germline editing has been proposed because the technology still lacks the required efficacy and safety. Here we focus on the approaches developed since 2013 to decrease the frequency of unwanted mutations (the off-targets) during CRISPR-based gene editing. Frontiers Media S.A. 2021-09-17 /pmc/articles/PMC8484971/ /pubmed/34604217 http://dx.doi.org/10.3389/fcell.2021.718466 Text en Copyright © 2021 Vicente, Chaves-Ferreira, Jorge, Proença and Barreto. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Vicente, Manuel M.
Chaves-Ferreira, Miguel
Jorge, João M. P.
Proença, João T.
Barreto, Vasco M.
The Off-Targets of Clustered Regularly Interspaced Short Palindromic Repeats Gene Editing
title The Off-Targets of Clustered Regularly Interspaced Short Palindromic Repeats Gene Editing
title_full The Off-Targets of Clustered Regularly Interspaced Short Palindromic Repeats Gene Editing
title_fullStr The Off-Targets of Clustered Regularly Interspaced Short Palindromic Repeats Gene Editing
title_full_unstemmed The Off-Targets of Clustered Regularly Interspaced Short Palindromic Repeats Gene Editing
title_short The Off-Targets of Clustered Regularly Interspaced Short Palindromic Repeats Gene Editing
title_sort off-targets of clustered regularly interspaced short palindromic repeats gene editing
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484971/
https://www.ncbi.nlm.nih.gov/pubmed/34604217
http://dx.doi.org/10.3389/fcell.2021.718466
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