Generating stable isolated mitochondrial recipient clones in mammalian cells using MitoPunch mitochondrial transfer

This protocol describes the assembly and use of MitoPunch to deliver mitochondria containing mitochondrial DNA (mtDNA) into cells lacking mtDNA (ρ0 cells). MitoPunch generates stable isolated mitochondrial recipient clones with restored mtDNA and recovered respiration, enabling investigation of mtDN...

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Detalles Bibliográficos
Autores principales: Sercel, Alexander J., Napior, Alexander J., Patananan, Alexander N., Wu, Ting-Hsiang, Chiou, Pei-Yu, Teitell, Michael A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8487092/
https://www.ncbi.nlm.nih.gov/pubmed/34632418
http://dx.doi.org/10.1016/j.xpro.2021.100850

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8487092/
https://www.ncbi.nlm.nih.gov/pubmed/34632418
http://dx.doi.org/10.1016/j.xpro.2021.100850

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