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Comparison of five polyethylene glycol precipitation procedures for the RT-qPCR based recovery of murine hepatitis virus, bacteriophage phi6, and pepper mild mottle virus as a surrogate for SARS-CoV-2 from wastewater

Polyethylene glycol (PEG) precipitation is one of the conventional methods for virus concentration. This technique has been used to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater. The procedures and seeded surrogate viruses were different among implementers; th...

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Detalles Bibliográficos
Autores principales: Torii, Shotaro, Oishi, Wakana, Zhu, Yifan, Thakali, Ocean, Malla, Bikash, Yu, Zaizhi, Zhao, Bo, Arakawa, Chisato, Kitajima, Masaaki, Hata, Akihiko, Ihara, Masaru, Kyuwa, Shigeru, Sano, Daisuke, Haramoto, Eiji, Katayama, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Author(s). Published by Elsevier B.V. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8487407/
https://www.ncbi.nlm.nih.gov/pubmed/34610400
http://dx.doi.org/10.1016/j.scitotenv.2021.150722