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Comparison of five polyethylene glycol precipitation procedures for the RT-qPCR based recovery of murine hepatitis virus, bacteriophage phi6, and pepper mild mottle virus as a surrogate for SARS-CoV-2 from wastewater
Polyethylene glycol (PEG) precipitation is one of the conventional methods for virus concentration. This technique has been used to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater. The procedures and seeded surrogate viruses were different among implementers; th...
Autores principales: | Torii, Shotaro, Oishi, Wakana, Zhu, Yifan, Thakali, Ocean, Malla, Bikash, Yu, Zaizhi, Zhao, Bo, Arakawa, Chisato, Kitajima, Masaaki, Hata, Akihiko, Ihara, Masaru, Kyuwa, Shigeru, Sano, Daisuke, Haramoto, Eiji, Katayama, Hiroyuki |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Author(s). Published by Elsevier B.V.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8487407/ https://www.ncbi.nlm.nih.gov/pubmed/34610400 http://dx.doi.org/10.1016/j.scitotenv.2021.150722 |
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