Flow-cytometric analysis of membrane integrity of stallion sperm in the face of agglutination: the “zombie sperm” dilemma

PURPOSE: To define the effect of sperm agglutination, associated with incubation under capacitating conditions, on accuracy of membrane assessment via flow cytometry and to develop methods to mitigate that effect. METHODS: Sperm motility was measured by CASA. Sperm were stained with PI-PSA or a nove...

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Detalles Bibliográficos
Autores principales: Ortiz, Isabel, Felix, Matheus, Resende, Hélène, Ramírez-Agámez, Luisa, Love, Charles C., Hinrichs, Katrin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Gamete Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490572/
https://www.ncbi.nlm.nih.gov/pubmed/33991296
http://dx.doi.org/10.1007/s10815-021-02134-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490572/
https://www.ncbi.nlm.nih.gov/pubmed/33991296
http://dx.doi.org/10.1007/s10815-021-02134-z

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