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Flow-cytometric analysis of membrane integrity of stallion sperm in the face of agglutination: the “zombie sperm” dilemma
PURPOSE: To define the effect of sperm agglutination, associated with incubation under capacitating conditions, on accuracy of membrane assessment via flow cytometry and to develop methods to mitigate that effect. METHODS: Sperm motility was measured by CASA. Sperm were stained with PI-PSA or a nove...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer US
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490572/ https://www.ncbi.nlm.nih.gov/pubmed/33991296 http://dx.doi.org/10.1007/s10815-021-02134-z |