Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search

RNA-guided Streptococcus pyogenes Cas9 (SpCas9) is a sequence-specific DNA endonuclease that works as one of the most powerful genetic editing tools. However, how Cas9 locates its target among huge amounts of dsDNAs remains elusive. Here, combining biochemical and single-molecule fluorescence assays...

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Detalles Bibliográficos
Autores principales: Yang, Mengyi, Sun, Ruirui, Deng, Pujuan, Yang, Yuzhuo, Wang, Wenjuan, Liu, Jun-Jie Gogo, Chen, Chunlai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8494019/
https://www.ncbi.nlm.nih.gov/pubmed/34703564
http://dx.doi.org/10.1039/d1sc02633j

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8494019/
https://www.ncbi.nlm.nih.gov/pubmed/34703564
http://dx.doi.org/10.1039/d1sc02633j

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