An efficient i-GONAD method for creating and maintaining lethal mutant mice using an inversion balancer identified from the C3H/HeJJcl strain

As the efficiency of the clustered regularly interspaced short palindromic repeats/Cas system is extremely high, creation and maintenance of homozygous lethal mutants are often difficult. Here, we present an efficient in vivo electroporation method called improved genome editing via oviductal nuclei...

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Detalles Bibliográficos
Autores principales: Iwata, Satoru, Sasaki, Takahisa, Nagahara, Miki, Iwamoto, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Investigation
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496231/
https://www.ncbi.nlm.nih.gov/pubmed/34849815
http://dx.doi.org/10.1093/g3journal/jkab194

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496231/
https://www.ncbi.nlm.nih.gov/pubmed/34849815
http://dx.doi.org/10.1093/g3journal/jkab194

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