DNA templates with blocked long 3ʹ end single-stranded overhangs (BL3SSO) promote bona fide Cas9-stimulated homology-directed repair of long transgenes into endogenous gene loci

Knock-in of large transgenes by Cas9-mediated homology-directed repair (HDR) is an extremely inefficient process. Although the use of single-stranded oligonucleotides (ssODN) as an HDR donor has improved the integration of smaller transgenes, they do not support efficient insertion of large DNA sequ...

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Detalles Bibliográficos
Autores principales: Bandyopadhyay, Saptaparni, Douglass, Joseph, Kapell, Sebastian, Khan, Nazimuddin, Feitosa-Suntheimer, Fabiana, Klein, Jenny A, Temple, Jasmine, Brown-Culbertson, Jayce, Tavares, Alexander H, Saeed, Mohsan, Lau, Nelson C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Investigation
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496256/
https://www.ncbi.nlm.nih.gov/pubmed/33989385
http://dx.doi.org/10.1093/g3journal/jkab169

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496256/
https://www.ncbi.nlm.nih.gov/pubmed/33989385
http://dx.doi.org/10.1093/g3journal/jkab169

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