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Generation of Drosophila attP containing cell lines using CRISPR-Cas9

The generation of Drosophila stable cell lines has become invaluable for complementing in vivo experiments and as tools for genetic screens. Recent advances utilizing attP/PhiC31 integrase system has permitted the creation of Drosophila cells in which recombination mediated cassette exchange (RMCE)...

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Detalles Bibliográficos
Autores principales: Mariyappa, Daniel, Luhur, Arthur, Overton, Danielle, Zelhof, Andrew C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496291/
https://www.ncbi.nlm.nih.gov/pubmed/33963853
http://dx.doi.org/10.1093/g3journal/jkab161