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Generation of Drosophila attP containing cell lines using CRISPR-Cas9
The generation of Drosophila stable cell lines has become invaluable for complementing in vivo experiments and as tools for genetic screens. Recent advances utilizing attP/PhiC31 integrase system has permitted the creation of Drosophila cells in which recombination mediated cassette exchange (RMCE)...
Autores principales: | Mariyappa, Daniel, Luhur, Arthur, Overton, Danielle, Zelhof, Andrew C |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496291/ https://www.ncbi.nlm.nih.gov/pubmed/33963853 http://dx.doi.org/10.1093/g3journal/jkab161 |
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