A protocol for rapid and parallel isolation of myocytes and non-myocytes from multiple mouse hearts

This protocol features parallel isolation of myocytes and non-myocytes from murine hearts. It was designed with considerations for (1) time required to extract cardiac cells, (2) cell viability, and (3) protocol scalability. Here, a peristaltic pump and 3D-printed elements are combined to perfuse th...

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Detalles Bibliográficos
Autores principales: Farrugia, Gabriella E., McLellan, Micheal A., Weeks, Kate L., Matsumoto, Aya, Cohen, Charles D., Krstevski, Crisdion, Gaynor, Taylah L., Parslow, Adam C., McMullen, Julie R., Pinto, Alexander R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496295/
https://www.ncbi.nlm.nih.gov/pubmed/34647038
http://dx.doi.org/10.1016/j.xpro.2021.100866

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496295/
https://www.ncbi.nlm.nih.gov/pubmed/34647038
http://dx.doi.org/10.1016/j.xpro.2021.100866

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