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Direct IgG epitope mapping on bacterial AB toxins by cryo-EM
This cryo-EM protocol was used to determine the B cell epitope map on the CdtB subunit of typhoid toxin, an A(2)B(5) toxin secreted by Salmonella Typhi during infection. Immunoglobulin G (IgG) was directly mixed with typhoid toxin in this protocol, different from our previous cryo-EM protocol that u...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496303/ https://www.ncbi.nlm.nih.gov/pubmed/34647035 http://dx.doi.org/10.1016/j.xpro.2021.100852 |