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Direct IgG epitope mapping on bacterial AB toxins by cryo-EM

This cryo-EM protocol was used to determine the B cell epitope map on the CdtB subunit of typhoid toxin, an A(2)B(5) toxin secreted by Salmonella Typhi during infection. Immunoglobulin G (IgG) was directly mixed with typhoid toxin in this protocol, different from our previous cryo-EM protocol that u...

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Detalles Bibliográficos
Autores principales: Nguyen, Tri, Song, Jeongmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496303/
https://www.ncbi.nlm.nih.gov/pubmed/34647035
http://dx.doi.org/10.1016/j.xpro.2021.100852