Direct IgG epitope mapping on bacterial AB toxins by cryo-EM

This cryo-EM protocol was used to determine the B cell epitope map on the CdtB subunit of typhoid toxin, an A(2)B(5) toxin secreted by Salmonella Typhi during infection. Immunoglobulin G (IgG) was directly mixed with typhoid toxin in this protocol, different from our previous cryo-EM protocol that u...

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Detalles Bibliográficos
Autores principales: Nguyen, Tri, Song, Jeongmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496303/
https://www.ncbi.nlm.nih.gov/pubmed/34647035
http://dx.doi.org/10.1016/j.xpro.2021.100852
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author Nguyen, Tri
Song, Jeongmin
author_facet Nguyen, Tri
Song, Jeongmin
author_sort Nguyen, Tri
collection PubMed
description This cryo-EM protocol was used to determine the B cell epitope map on the CdtB subunit of typhoid toxin, an A(2)B(5) toxin secreted by Salmonella Typhi during infection. Immunoglobulin G (IgG) was directly mixed with typhoid toxin in this protocol, different from our previous cryo-EM protocol that uses the Fab fragments in place of IgG. This simple approach requires smaller amounts of materials, supporting the broader use of this protocol for determining antibody recognition sites on various antigens. For complete details on the use and execution of this protocol, please refer to Ahn et al. (2021) and Nguyen et al. (2021).
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spelling pubmed-84963032021-10-12 Direct IgG epitope mapping on bacterial AB toxins by cryo-EM Nguyen, Tri Song, Jeongmin STAR Protoc Protocol This cryo-EM protocol was used to determine the B cell epitope map on the CdtB subunit of typhoid toxin, an A(2)B(5) toxin secreted by Salmonella Typhi during infection. Immunoglobulin G (IgG) was directly mixed with typhoid toxin in this protocol, different from our previous cryo-EM protocol that uses the Fab fragments in place of IgG. This simple approach requires smaller amounts of materials, supporting the broader use of this protocol for determining antibody recognition sites on various antigens. For complete details on the use and execution of this protocol, please refer to Ahn et al. (2021) and Nguyen et al. (2021). Elsevier 2021-10-01 /pmc/articles/PMC8496303/ /pubmed/34647035 http://dx.doi.org/10.1016/j.xpro.2021.100852 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Nguyen, Tri
Song, Jeongmin
Direct IgG epitope mapping on bacterial AB toxins by cryo-EM
title Direct IgG epitope mapping on bacterial AB toxins by cryo-EM
title_full Direct IgG epitope mapping on bacterial AB toxins by cryo-EM
title_fullStr Direct IgG epitope mapping on bacterial AB toxins by cryo-EM
title_full_unstemmed Direct IgG epitope mapping on bacterial AB toxins by cryo-EM
title_short Direct IgG epitope mapping on bacterial AB toxins by cryo-EM
title_sort direct igg epitope mapping on bacterial ab toxins by cryo-em
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496303/
https://www.ncbi.nlm.nih.gov/pubmed/34647035
http://dx.doi.org/10.1016/j.xpro.2021.100852
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