Cargando…

Sensitive ELISA-based detection method for the mitophagy marker p-S65-Ub in human cells, autopsy brain, and blood samples

Mitochondrial dysfunction is an early, imminent event in neurodegenerative disorders including Parkinson disease (PD) and Alzheimer disease (AD). The enzymatic pair PINK1 and PRKN/Parkin recognize and transiently label damaged mitochondria with ubiquitin (Ub) phosphorylated at Ser65 (p-S65-Ub) as a...

Descripción completa

Detalles Bibliográficos
Autores principales:	Watzlawik, Jens O., Hou, Xu, Fricova, Dominika, Ramnarine, Chloe, Barodia, Sandeep K., Gendron, Tania F., Heckman, Michael G., DeTure, Michael, Siuda, Joanna, Wszolek, Zbigniew K., Scherzer, Clemens R., Ross, Owen A., Bu, Guojun, Dickson, Dennis W., Goldberg, Matthew S., Fiesel, Fabienne C., Springer, Wolfdieter
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Taylor & Francis 2020
Materias:	Toolbox
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496550/ https://www.ncbi.nlm.nih.gov/pubmed/33112198 http://dx.doi.org/10.1080/15548627.2020.1834712

Ejemplares similares

A monolayer hiPSC culture system for autophagy/mitophagy studies in human dopaminergic neurons
por: Stathakos, Petros, et al.
Publicado: (2020)

Mitophagy alterations in Alzheimer's disease are associated with granulovacuolar degeneration and early tau pathology
por: Hou, Xu, et al.
Publicado: (2020)

Mitochondrial targeted HSP90 inhibitor Gamitrinib-TPP (G-TPP) induces PINK1/Parkin-dependent mitophagy
por: Fiesel, Fabienne C., et al.
Publicado: (2017)

High Data Output and Automated 3D Correlative Light–Electron Microscopy Method
por: Vicidomini, Giuseppe, et al.
Publicado: (2008)

A high-throughput FRET-based assay for determination of Atg4 activity
por: Li, Min, et al.
Publicado: (2012)

Semi-Automated Analysis of Organelle Movement and Membrane Content: Understanding Rab-Motor Complex Transport Function
por: Hume, Alistair N, et al.
Publicado: (2011)

A novel method for autophagy detection in primary cells: Impaired levels of macroautophagy in immunosenescent T cells
por: Phadwal, Kanchan, et al.
Publicado: (2012)

Live imaging and single-cell analysis reveal differential dynamics of autophagy and apoptosis
por: Xu, Yangqing, et al.
Publicado: (2013)

MitoTimer probe reveals the impact of autophagy, fusion, and motility on subcellular distribution of young and old mitochondrial protein and on relative mitochondrial protein age
por: Ferree, Andrew W, et al.
Publicado: (2013)

Correlative light and electron microscopy imaging of autophagy in a zebrafish infection model
por: Hosseini, Rohola, et al.
Publicado: (2014)

Dissecting Functions of the Conserved Oligomeric Golgi Tethering Complex Using a Cell-Free Assay
por: Cottam, Nathanael P, et al.
Publicado: (2014)

Protein Ligation in Living Cells Using Sortase
por: Strijbis, Karin, et al.
Publicado: (2012)

A rapid and high content assay that measures cyto-ID-stained autophagic compartments and estimates autophagy flux with potential clinical applications
por: Guo, Sujuan, et al.
Publicado: (2015)

Development and application of an antibody-based protein microarray to assess physiological stress in grizzly bears (Ursus arctos)
por: Carlson, Ruth I., et al.
Publicado: (2016)

Shotgun proteomics as a viable approach for biological discovery in the Pacific oyster
por: Timmins-Schiffman, Emma, et al.
Publicado: (2013)

Enzyme immunoassays as a method for quantifying hair reproductive hormones in two felid species
por: Terwissen, C. V., et al.
Publicado: (2014)

A practical field extraction method for non-invasive monitoring of hormone activity in the black rhinoceros
por: Edwards, Katie L., et al.
Publicado: (2014)

Effects of natural environmental conditions on faecal glucocorticoid metabolite concentrations in jaguars (Panthera onca) in Belize
por: Mesa-Cruz, J. Bernardo, et al.
Publicado: (2014)

Measurement of free glucocorticoids: quantifying corticosteroid-binding globulin binding affinity and its variation within and among mammalian species
por: Delehanty, Brendan, et al.
Publicado: (2015)

Using novel methodologies to examine the impact of artificial light at night on the cortisol stress response in dispersing Atlantic salmon (Salmo salar L.) fry
por: Newman, Rhian C., et al.
Publicado: (2015)

Developing a new research tool for use in free-ranging cetaceans: recovering cortisol from harbour porpoise skin
por: Bechshoft, Thea, et al.
Publicado: (2015)

Longitudinal progesterone profiles in baleen from female North Atlantic right whales (Eubalaena glacialis) match known calving history
por: Hunt, Kathleen E., et al.
Publicado: (2016)

Calibration of the HemoCue point-of-care analyser for determining haemoglobin concentration in a lizard and a fish
por: Andrewartha, Sarah J., et al.
Publicado: (2016)

Baleen hormones: a novel tool for retrospective assessment of stress and reproduction in bowhead whales (Balaena mysticetus)
por: Hunt, Kathleen E., et al.
Publicado: (2014)

Quantitative analysis of autophagic flux by confocal pH-imaging of autophagic intermediates
por: Maulucci, Giuseppe, et al.
Publicado: (2015)

Temporal overlap and repeatability of feather corticosterone levels: practical considerations for use as a biomarker
por: Harris, Christopher M., et al.
Publicado: (2016)

An evaluation of the use of pentosidine as a biomarker for ageing turtles
por: Iverson, John B., et al.
Publicado: (2017)

Effects of post-mortem storage conditions of bovine epididymides on sperm characteristics: investigating a tool for preservation of sperm from endangered species
por: Strand, Julie, et al.
Publicado: (2016)

Evaluating the effect of sample type on American alligator (Alligator mississippiensis) analyte values in a point-of-care blood analyser
por: Hamilton, Matthew T., et al.
Publicado: (2016)

Using HHsearch to tackle proteins of unknown function: A pilot study with PH domains
por: Fidler, David R., et al.
Publicado: (2016)

Three‐dimensional immersive virtual reality for studying cellular compartments in 3D models from EM preparations of neural tissues
por: Calì, Corrado, et al.
Publicado: (2016)

Get the most out of blow hormones: validation of sampling materials, field storage and extraction techniques for whale respiratory vapour samples
por: Burgess, Elizabeth A., et al.
Publicado: (2016)

Brain maps 4.0—Structure of the rat brain: An open access atlas with global nervous system nomenclature ontology and flatmaps
por: Swanson, Larry W.
Publicado: (2018)

Semen collection and ejaculate characteristics of the Leopard Tortoise (Stigmochelys pardalis)
por: Zimmerman, Dawn M, et al.
Publicado: (2017)

Multiple steroid and thyroid hormones detected in baleen from eight whale species
por: Hunt, Kathleen E, et al.
Publicado: (2017)

Within-sample variation in snowshoe hare faecal glucocorticoid metabolite measurements
por: Lafferty, Diana J R, et al.
Publicado: (2017)

Not just fat: investigating the proteome of cetacean blubber tissue
por: Kershaw, Joanna L, et al.
Publicado: (2018)

A novel method for the measurement of glucocorticoids in dermal secretions of amphibians
por: Santymire, R M, et al.
Publicado: (2018)

Using the shuttlebox experimental design to determine temperature preference for juvenile Westslope Cutthroat Trout (Oncorhynchus clarkii lewisi)
por: Macnaughton, Camille J, et al.
Publicado: (2018)

Generation of a sexually mature individual of the Eastern dwarf tree frog, Litoria fallax, from cryopreserved testicular macerates: proof of capacity of cryopreserved sperm derived offspring to complete development
por: Upton, Rose, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_lhdj0uk4m2lciju9sgf39puhb3