ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces

ssDNA recombineering has been exploited to hyperdiversify genomically-encoded nanobodies displayed on the surface of Escherichia coli for originating new binding properties. As a proof-of-principle a nanobody recognizing the antigen TirM from enterohaemorrhagic E. coli (EHEC) was evolved towards the...

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Detalles Bibliográficos
Autores principales: Al-ramahi, Yamal, Nyerges, Akos, Margolles, Yago, Cerdán, Lidia, Ferenc, Gyorgyi, Pál, Csaba, Fernández, Luis Ángel, de Lorenzo, Víctor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8497518/
https://www.ncbi.nlm.nih.gov/pubmed/34621006
http://dx.doi.org/10.1038/s42003-021-02702-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8497518/
https://www.ncbi.nlm.nih.gov/pubmed/34621006
http://dx.doi.org/10.1038/s42003-021-02702-0

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