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ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces

ssDNA recombineering has been exploited to hyperdiversify genomically-encoded nanobodies displayed on the surface of Escherichia coli for originating new binding properties. As a proof-of-principle a nanobody recognizing the antigen TirM from enterohaemorrhagic E. coli (EHEC) was evolved towards the...

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Detalles Bibliográficos
Autores principales:	Al-ramahi, Yamal, Nyerges, Akos, Margolles, Yago, Cerdán, Lidia, Ferenc, Gyorgyi, Pál, Csaba, Fernández, Luis Ángel, de Lorenzo, Víctor
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8497518/ https://www.ncbi.nlm.nih.gov/pubmed/34621006 http://dx.doi.org/10.1038/s42003-021-02702-0

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