Cargando…

Development and characterization of phasor-based analysis for FLIM to evaluate the metabolic and epigenetic impact of HER2 inhibition on squamous cell carcinoma cultures

Significance: Deranged metabolism and dysregulated growth factor signaling are closely associated with abnormal levels of proliferation, a recognized hallmark in tumorigenesis. Fluorescence lifetime imaging microscopy (FLIM) of endogenous nicotinamide adenine dinucleotide (NADH), a key metabolic coe...

Descripción completa

Detalles Bibliográficos
Autores principales:	Pham, Dan L., Miller, Christina R., Myers, Molly S., Myers, Dominick M., Hansen, Laura A., Nichols, Michael G.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Society of Photo-Optical Instrumentation Engineers 2021
Materias:	Microscopy
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8501457/ https://www.ncbi.nlm.nih.gov/pubmed/34628733 http://dx.doi.org/10.1117/1.JBO.26.10.106501

Ejemplares similares

Automatic detection of head and neck squamous cell carcinoma on histologic slides using hyperspectral microscopic imaging
por: Ma, Ling, et al.
Publicado: (2022)

Phasor identifier: A cloud-based analysis of phasor-FLIM data on Python notebooks
por: Bernardi, Mario, et al.
Publicado: (2023)

In vivo melanin 3D quantification and z-epidermal distribution by multiphoton FLIM, phasor and Pseudo-FLIM analyses
por: Pena, Ana-Maria, et al.
Publicado: (2022)

Wide-field time-gated SPAD imager for phasor-based FLIM applications
por: Ulku, Arin, et al.
Publicado: (2020)

Applying phasor approach analysis of multiphoton FLIM measurements to probe the metabolic activity of three-dimensional in vitro cell culture models
por: Lakner, Pirmin H., et al.
Publicado: (2017)

Phasor analysis of NADH FLIM identifies pharmacological disruptions to mitochondrial metabolic processes in the rodent cerebral cortex
por: Gómez, Carlos A., et al.
Publicado: (2018)

Extracellular pH affects the fluorescence lifetimes of metabolic co-factors
por: Schmitz, Rebecca, et al.
Publicado: (2021)

The phasor-FLIM fingerprints reveal shifts from OXPHOS to enhanced glycolysis in Huntington Disease
por: Sameni, Sara, et al.
Publicado: (2016)

Dissecting aggregation and seeding dynamics of α-Syn polymorphs using the phasor approach to FLIM
por: Tittelmeier, Jessica, et al.
Publicado: (2022)

Metabolic mapping of glioblastoma stem cells reveals NADH fluxes associated with glioblastoma phenotype and survival
por: Schroeder, Alexandra B., et al.
Publicado: (2020)

Off-axis quantitative phase imaging processing using CUDA: toward real-time applications
por: Pham, Hoa, et al.
Publicado: (2011)

Label-free assessment of pre-implantation embryo quality by the Fluorescence Lifetime Imaging Microscopy (FLIM)-phasor approach
por: Ma, Ning, et al.
Publicado: (2019)

Characterization and identification of cell death dynamics by quantitative phase imaging
por: Hsieh, Huai-Ching, et al.
Publicado: (2022)

Quantitative dispersion microscopy
por: Fu, Dan, et al.
Publicado: (2010)

Multimodal wide-field two-photon excitation imaging: characterization of the technique for in vivo applications
por: Hwang, Jae Youn, et al.
Publicado: (2011)

Propagating-path uniformly scanned light sheet excitation microscopy for isotropic volumetric imaging of large specimens
por: Ping, Junyu, et al.
Publicado: (2019)

Automatic detection and characterization of quantitative phase images of thalassemic red blood cells using a mask region-based convolutional neural network
por: Lin, Yang-Hsien, et al.
Publicado: (2020)

Label-free characterization of living human induced pluripotent stem cells by subcellular topographic imaging technique using full-field quantitative phase microscopy coupled with interference reflection microscopy
por: Sugiyama, Norikazu, et al.
Publicado: (2012)

Alpha Hemolysin Induces an Increase of Erythrocytes Calcium: A FLIM 2-Photon Phasor Analysis Approach
por: Sanchez, Susana, et al.
Publicado: (2011)

Particle-based phasor-FLIM-FRET resolves protein-protein interactions inside single viral particles
por: Coucke, Quinten, et al.
Publicado: (2023)

Caffeine and Cisplatin Effectively Targets the Metabolism of a Triple-Negative Breast Cancer Cell Line Assessed via Phasor-FLIM
por: Pascua, Stephanie M., et al.
Publicado: (2020)

Cell imaging beyond the diffraction limit using sparse deconvolution spatial light interference microscopy
por: Babacan, S. Derin, et al.
Publicado: (2011)

Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens
por: Grewe, Benjamin F., et al.
Publicado: (2011)

Performance of full-pupil line-scanning reflectance confocal microscopy in human skin and oral mucosa in vivo
por: Larson, Bjorg, et al.
Publicado: (2011)

A cylindrical zoom lens unit for adjustable optical sectioning in light sheet microscopy
por: Ritter, Jörg G., et al.
Publicado: (2010)

The structural origin of second harmonic generation in fascia
por: Rivard, Maxime, et al.
Publicado: (2010)

Sub-wavelength infrared imaging of lipids
por: Yarrow, Fiona, et al.
Publicado: (2010)

Contrast improvement of terahertz images of thin histopathologic sections
por: Formanek, Florian, et al.
Publicado: (2010)

High speed multiphoton axial scanning through an optical fiber in a remotely scanned temporal focusing setup
por: Straub, Adam, et al.
Publicado: (2010)

Remote focusing for programmable multi-layer differential multiphoton microscopy
por: Hoover, Erich E., et al.
Publicado: (2010)

Combined third-harmonic generation and four-wave mixing microscopy of tissues and embryos
por: Mahou, Pierre, et al.
Publicado: (2011)

Fast macro-scale transmission imaging of microvascular networks using KESM
por: Mayerich, David, et al.
Publicado: (2011)

CARS microscopy for the visualization of micrometer-sized iron oxide MRI contrast agents in living cells
por: Rago, Gianluca, et al.
Publicado: (2011)

Combined confocal Raman and quantitative phase microscopy system for biomedical diagnosis
por: Kang, Jeon Woong, et al.
Publicado: (2011)

Evaluation of a spectrally resolved scattering microscope
por: Schmitz, Michael, et al.
Publicado: (2011)

Field-portable reflection and transmission microscopy based on lensless holography
por: Lee, Myungjun, et al.
Publicado: (2011)

Fluorescence microspectroscopy as a tool to study mechanism of nanoparticles delivery into living cancer cells
por: Arsov, Zoran, et al.
Publicado: (2011)

Chemoselective imaging of mouse brain tissue via multiplex CARS microscopy
por: Pohling, Christoph, et al.
Publicado: (2011)

Differential diagnosis of breast cancer using quantitative, label-free and molecular vibrational imaging
por: Yang, Yaliang, et al.
Publicado: (2011)

Optimization of pupil design for point-scanning and line-scanning confocal microscopy
por: Patel, Yogesh G., et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_mp7udrhj0et64r16j684s0ac8m