Cargando…

Optimized nickase- and nuclease-based prime editing in human and mouse cells

Precise genomic modification using prime editing (PE) holds enormous potential for research and clinical applications. In this study, we generated all-in-one prime editing (PEA1) constructs that carry all the components required for PE, along with a selection marker. We tested these constructs (with...

Descripción completa

Detalles Bibliográficos
Autores principales:	Adikusuma, Fatwa, Lushington, Caleb, Arudkumar, Jayshen, Godahewa, Gelshan I, Chey, Yu C J, Gierus, Luke, Piltz, Sandra, Geiger, Ashleigh, Jain, Yatish, Reti, Daniel, Wilson, Laurence O W, Bauer, Denis C, Thomas, Paul Q
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2021
Materias:	Synthetic Biology and Bioengineering
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8501948/ https://www.ncbi.nlm.nih.gov/pubmed/34534334 http://dx.doi.org/10.1093/nar/gkab792

Ejemplares similares

CRISPR applications for Duchenne muscular dystrophy: From animal models to potential therapies
por: Chey, Yu C. J., et al.
Publicado: (2022)

Precise and broad scope genome editing based on high-specificity Cas9 nickases
por: Wang, Qian, et al.
Publicado: (2021)

Unbiased investigation of specificities of prime editing systems in human cells
por: Kim, Do Yon, et al.
Publicado: (2020)

Comprehensive analysis of prime editing outcomes in human embryonic stem cells
por: Habib, Omer, et al.
Publicado: (2022)

Engineered lentivirus-derived nanoparticles (LVNPs) for delivery of CRISPR/Cas ribonucleoprotein complexes supporting base editing, prime editing and in vivo gene modification
por: Haldrup, Jakob, et al.
Publicado: (2023)

Large-scale genome editing based on high-capacity adenovectors and CRISPR-Cas9 nucleases rescues full-length dystrophin synthesis in DMD muscle cells
por: Tasca, Francesca, et al.
Publicado: (2022)

The contribution of homology arms to nuclease-assisted genome engineering
por: Baker, Oliver, et al.
Publicado: (2017)

Highly efficient CRISPR-mediated large DNA docking and multiplexed prime editing using a single baculovirus
por: Aulicino, Francesco, et al.
Publicado: (2022)

Nuclease-mediated gene editing by homologous recombination of the human globin locus
por: Voit, Richard A., et al.
Publicado: (2014)

Prime editing with genuine Cas9 nickases minimizes unwanted indels
por: Lee, Jaesuk, et al.
Publicado: (2023)

Enhancing site-specific DNA integration by a Cas9 nuclease fused with a DNA donor-binding domain
por: Ma, Shufeng, et al.
Publicado: (2020)

Selection-free gene repair after adenoviral vector transduction of designer nucleases: rescue of dystrophin synthesis in DMD muscle cell populations
por: Maggio, Ignazio, et al.
Publicado: (2016)

Probing the impact of chromatin conformation on genome editing tools
por: Chen, Xiaoyu, et al.
Publicado: (2016)

Specific targeting of plasmids with Argonaute enables genome editing
por: Esyunina, Daria, et al.
Publicado: (2023)

Translational enhancement by base editing of the Kozak sequence rescues haploinsufficiency
por: Ambrosini, Chiara, et al.
Publicado: (2022)

Strategies to identify and edit improvements in synthetic genome segments episomally
por: Rudolph, Alexandra, et al.
Publicado: (2023)

A versatile platform strain for high-fidelity multiplex genome editing
por: Egbert, Robert G, et al.
Publicado: (2019)

Predicting base editing outcomes using position-specific sequence determinants
por: Pallaseni, Ananth, et al.
Publicado: (2022)

Retron-mediated multiplex genome editing and continuous evolution in Escherichia coli
por: Liu, Wenqian, et al.
Publicado: (2023)

Enabling large-scale genome editing at repetitive elements by reducing DNA nicking
por: Smith, Cory J, et al.
Publicado: (2020)

Small-molecule compounds boost genome-editing efficiency of cytosine base editor
por: Zhao, Tianyuan, et al.
Publicado: (2021)

Programmable RNA base editing with a single gRNA-free enzyme
por: Han, Wenjian, et al.
Publicado: (2022)

Parallelized gene cluster editing illuminates mechanisms of epoxyketone proteasome inhibitor biosynthesis
por: Huang, Chuan, et al.
Publicado: (2023)

Suppression of HBV replication by the expression of nickase- and nuclease dead-Cas9
por: Kurihara, Takeshi, et al.
Publicado: (2017)

Improvements of nuclease and nickase gene modification techniques for the treatment of genetic diseases
por: Lu, Yaoyao, et al.
Publicado: (2022)

Broadening the reach and investigating the potential of prime editors through fully viral gene-deleted adenoviral vector delivery
por: Wang, Qian, et al.
Publicado: (2021)

FnCpf1: a novel and efficient genome editing tool for Saccharomyces cerevisiae
por: Świat, Michal A., et al.
Publicado: (2017)

Small-molecule inhibitors of histone deacetylase improve CRISPR-based adenine base editing
por: Shin, Ha Rim, et al.
Publicado: (2021)

CRISPR–Cas12a system in fission yeast for multiplex genomic editing and CRISPR interference
por: Zhao, Yu, et al.
Publicado: (2020)

Rational guide RNA engineering for small-molecule control of CRISPR/Cas9 and gene editing
por: Liu, Xingyu, et al.
Publicado: (2022)

Imperfect guide-RNA (igRNA) enables CRISPR single-base editing with ABE and CBE
por: Zhao, Dongdong, et al.
Publicado: (2022)

Enhancing multiplex genome editing by natural transformation (MuGENT) via inactivation of ssDNA exonucleases
por: Dalia, Triana N., et al.
Publicado: (2017)

Characterization of a promiscuous DNA sulfur binding domain and application in site-directed RNA base editing
por: Hu, Wenyue, et al.
Publicado: (2023)

Precise homology-directed installation of large genomic edits in human cells with cleaving and nicking high-specificity Cas9 variants
por: Wang, Qian, et al.
Publicado: (2023)

A novel Cas9 fusion protein promotes targeted genome editing with reduced mutational burden in primary human cells
por: Carusillo, Antonio, et al.
Publicado: (2023)

Precise genome editing of the Kozak sequence enables bidirectional and quantitative modulation of protein translation to anticipated levels without affecting transcription
por: Xie, Jingke, et al.
Publicado: (2023)

Expanding the editable genome and CRISPR–Cas9 versatility using DNA cutting-free gene targeting based on in trans paired nicking
por: Chen, Xiaoyu, et al.
Publicado: (2020)

A functional type II-A CRISPR–Cas system from Listeria enables efficient genome editing of large non-integrating bacteriophage
por: Hupfeld, Mario, et al.
Publicado: (2018)

AGBE: a dual deaminase-mediated base editor by fusing CGBE with ABE for creating a saturated mutant population with multiple editing patterns
por: Liang, Yanhui, et al.
Publicado: (2022)

Highly efficient ‘hit-and-run’ genome editing with unconcentrated lentivectors carrying Vpr.Prot.Cas9 protein produced from RRE-containing transcripts
por: Indikova, Ivana, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_0qc05p6d15ts1cpdhpfgrf17ku