Timing and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events

OBJECTIVE: Lipofection-mediated introduction of the CRISPR/Cas9 system in porcine zygotes provides a simple method for gene editing, without requiring micromanipulation. However, the gene editing efficiency is inadequate. The aim of this study was to improve the lipofection-mediated gene editing eff...

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Detalles Bibliográficos
Autores principales: Lin, Qingyi, Le, Quynh Anh, Takebayashi, Koki, Thongkittidilok, Chommanart, Wittayarat, Manita, Hirata, Maki, Tanihara, Fuminori, Otoi, Takeshige
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8502333/
https://www.ncbi.nlm.nih.gov/pubmed/34627381
http://dx.doi.org/10.1186/s13104-021-05800-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8502333/
https://www.ncbi.nlm.nih.gov/pubmed/34627381
http://dx.doi.org/10.1186/s13104-021-05800-8

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