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Timing and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events

OBJECTIVE: Lipofection-mediated introduction of the CRISPR/Cas9 system in porcine zygotes provides a simple method for gene editing, without requiring micromanipulation. However, the gene editing efficiency is inadequate. The aim of this study was to improve the lipofection-mediated gene editing eff...

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Detalles Bibliográficos
Autores principales:	Lin, Qingyi, Le, Quynh Anh, Takebayashi, Koki, Thongkittidilok, Chommanart, Wittayarat, Manita, Hirata, Maki, Tanihara, Fuminori, Otoi, Takeshige
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2021
Materias:	Research Note
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8502333/ https://www.ncbi.nlm.nih.gov/pubmed/34627381 http://dx.doi.org/10.1186/s13104-021-05800-8

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