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microRNA-mediated regulation of microRNA machinery controls cell fate decisions
microRNAs associate with Argonaute proteins, forming the microRNA-induced silencing complex (miRISC), to repress target gene expression post-transcriptionally. Although microRNAs are critical regulators in mammalian cell differentiation, our understanding of how microRNA machinery, such as the miRIS...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8504967/ https://www.ncbi.nlm.nih.gov/pubmed/34596044 http://dx.doi.org/10.7554/eLife.72289 |
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author | Liu, Qiuying Novak, Mariah K Pepin, Rachel M Eich, Taylor Hu, Wenqian |
author_facet | Liu, Qiuying Novak, Mariah K Pepin, Rachel M Eich, Taylor Hu, Wenqian |
author_sort | Liu, Qiuying |
collection | PubMed |
description | microRNAs associate with Argonaute proteins, forming the microRNA-induced silencing complex (miRISC), to repress target gene expression post-transcriptionally. Although microRNAs are critical regulators in mammalian cell differentiation, our understanding of how microRNA machinery, such as the miRISC, are regulated during development is still limited. We previously showed that repressing the production of one Argonaute protein, Ago2, by Trim71 is important for mouse embryonic stem cells (mESCs) self-renewal (Liu et al., 2021). Here, we show that among the four Argonaute proteins in mammals, Ago2 is the major developmentally regulated Argonaute protein in mESCs. Moreover, in pluripotency, besides the Trim71-mediated regulation of Ago2 (Liu et al., 2021), Mir182/Mir183 also repress Ago2. Specific inhibition of this microRNA-mediated repression results in stemness defects and accelerated differentiation through the let-7 microRNA pathway. These results reveal a microRNA-mediated regulatory circuit on microRNA machinery that is critical to maintaining pluripotency. |
format | Online Article Text |
id | pubmed-8504967 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-85049672021-10-13 microRNA-mediated regulation of microRNA machinery controls cell fate decisions Liu, Qiuying Novak, Mariah K Pepin, Rachel M Eich, Taylor Hu, Wenqian eLife Cell Biology microRNAs associate with Argonaute proteins, forming the microRNA-induced silencing complex (miRISC), to repress target gene expression post-transcriptionally. Although microRNAs are critical regulators in mammalian cell differentiation, our understanding of how microRNA machinery, such as the miRISC, are regulated during development is still limited. We previously showed that repressing the production of one Argonaute protein, Ago2, by Trim71 is important for mouse embryonic stem cells (mESCs) self-renewal (Liu et al., 2021). Here, we show that among the four Argonaute proteins in mammals, Ago2 is the major developmentally regulated Argonaute protein in mESCs. Moreover, in pluripotency, besides the Trim71-mediated regulation of Ago2 (Liu et al., 2021), Mir182/Mir183 also repress Ago2. Specific inhibition of this microRNA-mediated repression results in stemness defects and accelerated differentiation through the let-7 microRNA pathway. These results reveal a microRNA-mediated regulatory circuit on microRNA machinery that is critical to maintaining pluripotency. eLife Sciences Publications, Ltd 2021-10-01 /pmc/articles/PMC8504967/ /pubmed/34596044 http://dx.doi.org/10.7554/eLife.72289 Text en © 2021, Liu et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Liu, Qiuying Novak, Mariah K Pepin, Rachel M Eich, Taylor Hu, Wenqian microRNA-mediated regulation of microRNA machinery controls cell fate decisions |
title | microRNA-mediated regulation of microRNA machinery controls cell fate decisions |
title_full | microRNA-mediated regulation of microRNA machinery controls cell fate decisions |
title_fullStr | microRNA-mediated regulation of microRNA machinery controls cell fate decisions |
title_full_unstemmed | microRNA-mediated regulation of microRNA machinery controls cell fate decisions |
title_short | microRNA-mediated regulation of microRNA machinery controls cell fate decisions |
title_sort | microrna-mediated regulation of microrna machinery controls cell fate decisions |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8504967/ https://www.ncbi.nlm.nih.gov/pubmed/34596044 http://dx.doi.org/10.7554/eLife.72289 |
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