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Using Cy5-dUTP labelling of RPA-amplicons with downstream microarray analysis for the detection of antibiotic resistance genes

In this report we describe Cy5-dUTP labelling of recombinase-polymerase-amplification (RPA) products directly during the amplification process for the first time. Nucleic acid amplification techniques, especially polymerase-chain-reaction as well as various isothermal amplification methods such as R...

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Detalles Bibliográficos
Autores principales: Warmt, Christian, Fenzel, Carolin Kornelia, Henkel, Jörg, Bier, Frank Fabian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8505619/
https://www.ncbi.nlm.nih.gov/pubmed/34635776
http://dx.doi.org/10.1038/s41598-021-99774-z