Cargando…
Using Cy5-dUTP labelling of RPA-amplicons with downstream microarray analysis for the detection of antibiotic resistance genes
In this report we describe Cy5-dUTP labelling of recombinase-polymerase-amplification (RPA) products directly during the amplification process for the first time. Nucleic acid amplification techniques, especially polymerase-chain-reaction as well as various isothermal amplification methods such as R...
Autores principales: | Warmt, Christian, Fenzel, Carolin Kornelia, Henkel, Jörg, Bier, Frank Fabian |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8505619/ https://www.ncbi.nlm.nih.gov/pubmed/34635776 http://dx.doi.org/10.1038/s41598-021-99774-z |
Ejemplares similares
-
An experimental comparison between primer and nucleotide labelling to produce RPA-amplicons used for multiplex detection of antibiotic resistance genes
por: Warmt, Christian, et al.
Publicado: (2023) -
Lateral flow–based nucleic acid detection of SARS-CoV-2 using enzymatic incorporation of biotin-labeled dUTP for POCT use
por: Agarwal, Saloni, et al.
Publicado: (2022) -
Preamplification with dUTP and Cod UNG Enables Elimination of Contaminating Amplicons
por: Andersson, Daniel, et al.
Publicado: (2018) -
dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase
por: Zasedateleva, Olga A, et al.
Publicado: (2018) -
dUTP incorporation into genomic DNA is linked to transcription in yeast
por: Kim, Nayun, et al.
Publicado: (2009)