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Optimized expression and purification of adipose triglyceride lipase improved hydrolytic and transacylation activities in vitro

Adipose triglyceride lipase (ATGL) plays a key role in intracellular lipolysis, the mobilization of stored triacylglycerol. This work provides an important basis for generating reproducible and detailed data on the hydrolytic and transacylation activities of ATGL. We generated full-length and C-term...

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Detalles Bibliográficos
Autores principales: Kulminskaya, Natalia, Radler, Claudia, Viertlmayr, Roland, Heier, Christoph, Hofer, Peter, Colaço-Gaspar, Mariana, Owens, Raymond J., Zimmermann, Robert, Schreiber, Renate, Zechner, Rudolf, Oberer, Monika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8506970/
https://www.ncbi.nlm.nih.gov/pubmed/34543623
http://dx.doi.org/10.1016/j.jbc.2021.101206