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Optimized expression and purification of adipose triglyceride lipase improved hydrolytic and transacylation activities in vitro
Adipose triglyceride lipase (ATGL) plays a key role in intracellular lipolysis, the mobilization of stored triacylglycerol. This work provides an important basis for generating reproducible and detailed data on the hydrolytic and transacylation activities of ATGL. We generated full-length and C-term...
Autores principales: | Kulminskaya, Natalia, Radler, Claudia, Viertlmayr, Roland, Heier, Christoph, Hofer, Peter, Colaço-Gaspar, Mariana, Owens, Raymond J., Zimmermann, Robert, Schreiber, Renate, Zechner, Rudolf, Oberer, Monika |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Biochemistry and Molecular Biology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8506970/ https://www.ncbi.nlm.nih.gov/pubmed/34543623 http://dx.doi.org/10.1016/j.jbc.2021.101206 |
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