Multiplex Site-Directed Gene Editing Using Polyethylene Glycol-Mediated Delivery of CRISPR gRNA:Cas9 Ribonucleoprotein (RNP) Complexes to Carrot Protoplasts

The aim of this work was to show an efficient, recombinant DNA-free, multiplex gene-editing method using gRNA:Cas9 ribonucleoprotein (RNP) complexes delivered directly to plant protoplasts. For this purpose, three RNPs were formed in the tube, their activity was confirmed by DNA cleavage in vitro, a...

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Detalles Bibliográficos
Autores principales: Klimek-Chodacka, Magdalena, Gieniec, Miron, Baranski, Rafal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8509836/
https://www.ncbi.nlm.nih.gov/pubmed/34639081
http://dx.doi.org/10.3390/ijms221910740

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8509836/
https://www.ncbi.nlm.nih.gov/pubmed/34639081
http://dx.doi.org/10.3390/ijms221910740

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