Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli

CRISPR interference (CRISPRi) has facilitated the study of essential genes in diverse organisms using both high-throughput and targeted approaches. Despite the promise of this technique, no comprehensive arrayed CRISPRi library targeting essential genes exists for the model bacterium Escherichia col...

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Detalles Bibliográficos
Autores principales: Silvis, Melanie R., Rajendram, Manohary, Shi, Handuo, Osadnik, Hendrik, Gray, Andrew N., Cesar, Spencer, Peters, Jason M., Hearne, Cameron C., Kumar, Parth, Todor, Horia, Huang, Kerwyn Casey, Gross, Carol A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8510551/
https://www.ncbi.nlm.nih.gov/pubmed/34634934
http://dx.doi.org/10.1128/mBio.02561-21

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8510551/
https://www.ncbi.nlm.nih.gov/pubmed/34634934
http://dx.doi.org/10.1128/mBio.02561-21

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