Generation of scalable cancer models by combining AAV-intron-trap, CRISPR/Cas9, and inducible Cre-recombinase

Scalable isogenic models of cancer-associated mutations are critical to studying dysregulated gene function. Nonsynonymous mutations of splicing factors, which typically affect one allele, are common in many cancers, but paradoxically confer growth disadvantage to cell lines, making their generation...

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Detalles Bibliográficos
Autores principales: Boddu, Prajwal C., Gupta, Abhishek K., Kim, Jung-Sik, Neugebauer, Karla M., Waldman, Todd, Pillai, Manoj M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8514589/
https://www.ncbi.nlm.nih.gov/pubmed/34645977
http://dx.doi.org/10.1038/s42003-021-02690-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8514589/
https://www.ncbi.nlm.nih.gov/pubmed/34645977
http://dx.doi.org/10.1038/s42003-021-02690-1

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