Lipidomics analysis of human follicular fluid form normal-weight patients with polycystic ovary syndrome: a pilot study

BACKGROUND: The polycystic ovary syndrome (PCOS) is the most common endocrine associated with insulin resistance, even in the absence of overweight. The global lipid profile of the follicular fluid in PCOS with normal weight as yet has not been investigated. The objection of this pilot study was to...

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Autores principales: Ban, Yanna, Ran, Haiying, Chen, Ying, Ma, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515674/
https://www.ncbi.nlm.nih.gov/pubmed/34645507
http://dx.doi.org/10.1186/s13048-021-00885-y
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author Ban, Yanna
Ran, Haiying
Chen, Ying
Ma, Li
author_facet Ban, Yanna
Ran, Haiying
Chen, Ying
Ma, Li
author_sort Ban, Yanna
collection PubMed
description BACKGROUND: The polycystic ovary syndrome (PCOS) is the most common endocrine associated with insulin resistance, even in the absence of overweight. The global lipid profile of the follicular fluid in PCOS with normal weight as yet has not been investigated. The objection of this pilot study was to explore the changes of lipids in the follicular fluid of PCOS with normal weight. METHODS: Follicular fluid samples were collected from patients who underwent IVF, including normal-weight women without PCOS (control group, n = 10) and normal-weight women with PCOS (PCOS group, n = 8). A lipidomic analysis was performed by high performance liquid chromatography/ mass spectrometry (HPLC-MS). Multidimensional statistical analysis was performed to disclose the global differences between the two groups. Further, differential lipid analysis between the two groups was performed by Fold Change Analysis (FC Analysis) and T-test to screen potential markers. RESULTS: All 812 species of 32 subclasses of lipids were identified by lipidomics analysis. 108 kinds of lipids were considered as the potential candidate differential metabolites with the score of variable importance in the project (VIP) more than 1 by the orthogonal partial least squares discriminant analysis. 32 lipids were significantly different between the PCOS group and the control group simultaneously with FC > 1.5 or FC < 0.67, p-value < 0.05 and VIP value > 1. These differential species of lipid belong to lipid subclasses including triglycerides (TG), phosphatidylethanolamines (PE) and phosphatidylinositols (PI). CONCLUSION: The identified differential lipids in the follicular fluid may be considered as candidate biomarkers as well as therapeutic targets of PCOS with normal-weight. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13048-021-00885-y.
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spelling pubmed-85156742021-10-20 Lipidomics analysis of human follicular fluid form normal-weight patients with polycystic ovary syndrome: a pilot study Ban, Yanna Ran, Haiying Chen, Ying Ma, Li J Ovarian Res Research BACKGROUND: The polycystic ovary syndrome (PCOS) is the most common endocrine associated with insulin resistance, even in the absence of overweight. The global lipid profile of the follicular fluid in PCOS with normal weight as yet has not been investigated. The objection of this pilot study was to explore the changes of lipids in the follicular fluid of PCOS with normal weight. METHODS: Follicular fluid samples were collected from patients who underwent IVF, including normal-weight women without PCOS (control group, n = 10) and normal-weight women with PCOS (PCOS group, n = 8). A lipidomic analysis was performed by high performance liquid chromatography/ mass spectrometry (HPLC-MS). Multidimensional statistical analysis was performed to disclose the global differences between the two groups. Further, differential lipid analysis between the two groups was performed by Fold Change Analysis (FC Analysis) and T-test to screen potential markers. RESULTS: All 812 species of 32 subclasses of lipids were identified by lipidomics analysis. 108 kinds of lipids were considered as the potential candidate differential metabolites with the score of variable importance in the project (VIP) more than 1 by the orthogonal partial least squares discriminant analysis. 32 lipids were significantly different between the PCOS group and the control group simultaneously with FC > 1.5 or FC < 0.67, p-value < 0.05 and VIP value > 1. These differential species of lipid belong to lipid subclasses including triglycerides (TG), phosphatidylethanolamines (PE) and phosphatidylinositols (PI). CONCLUSION: The identified differential lipids in the follicular fluid may be considered as candidate biomarkers as well as therapeutic targets of PCOS with normal-weight. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13048-021-00885-y. BioMed Central 2021-10-13 /pmc/articles/PMC8515674/ /pubmed/34645507 http://dx.doi.org/10.1186/s13048-021-00885-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Ban, Yanna
Ran, Haiying
Chen, Ying
Ma, Li
Lipidomics analysis of human follicular fluid form normal-weight patients with polycystic ovary syndrome: a pilot study
title Lipidomics analysis of human follicular fluid form normal-weight patients with polycystic ovary syndrome: a pilot study
title_full Lipidomics analysis of human follicular fluid form normal-weight patients with polycystic ovary syndrome: a pilot study
title_fullStr Lipidomics analysis of human follicular fluid form normal-weight patients with polycystic ovary syndrome: a pilot study
title_full_unstemmed Lipidomics analysis of human follicular fluid form normal-weight patients with polycystic ovary syndrome: a pilot study
title_short Lipidomics analysis of human follicular fluid form normal-weight patients with polycystic ovary syndrome: a pilot study
title_sort lipidomics analysis of human follicular fluid form normal-weight patients with polycystic ovary syndrome: a pilot study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515674/
https://www.ncbi.nlm.nih.gov/pubmed/34645507
http://dx.doi.org/10.1186/s13048-021-00885-y
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