Shared Mutations in Emerging SARS-CoV-2 Circulating Variants May Lead to Reverse Transcription-PCR (RT-PCR)-Based Misidentification of B.1.351 and P.1 Variants of Concern

Reverse transcription-PCRs (RT-PCRs) targeting SARS-CoV-2 variant of concern (VOC) mutations have been developed to simplify their tracking. We evaluated an assay targeting E484K/N501Y to identify B.1.351/P1. Whole-genome sequencing (WGS) confirmed only 72 (59.02%) of 122 consecutive RT-PCR P.1/B.1....

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Autores principales: Losada, Carmen, Rico-Luna, Carla, Otero-Sobrino, Álvaro, Molero-Salinas, Andrea, Buenestado-Serrano, Sergio, Candela, Ana, Pérez-Lago, Laura, Muñoz, Patricia, Catalán, Pilar, de Viedma, Darío García
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Observation
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515932/
https://www.ncbi.nlm.nih.gov/pubmed/34643437
http://dx.doi.org/10.1128/Spectrum.00816-21
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author Losada, Carmen
Rico-Luna, Carla
Otero-Sobrino, Álvaro
Molero-Salinas, Andrea
Buenestado-Serrano, Sergio
Candela, Ana
Pérez-Lago, Laura
Muñoz, Patricia
Catalán, Pilar
de Viedma, Darío García
author_facet Losada, Carmen
Rico-Luna, Carla
Otero-Sobrino, Álvaro
Molero-Salinas, Andrea
Buenestado-Serrano, Sergio
Candela, Ana
Pérez-Lago, Laura
Muñoz, Patricia
Catalán, Pilar
de Viedma, Darío García
author_sort Losada, Carmen
collection PubMed
description Reverse transcription-PCRs (RT-PCRs) targeting SARS-CoV-2 variant of concern (VOC) mutations have been developed to simplify their tracking. We evaluated an assay targeting E484K/N501Y to identify B.1.351/P1. Whole-genome sequencing (WGS) confirmed only 72 (59.02%) of 122 consecutive RT-PCR P.1/B.1.351 candidates. Prescreening RT-PCRs must target a wider set of mutations, updated from WGS data from emerging variants.
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spelling pubmed-85159322021-11-08 Shared Mutations in Emerging SARS-CoV-2 Circulating Variants May Lead to Reverse Transcription-PCR (RT-PCR)-Based Misidentification of B.1.351 and P.1 Variants of Concern Losada, Carmen Rico-Luna, Carla Otero-Sobrino, Álvaro Molero-Salinas, Andrea Buenestado-Serrano, Sergio Candela, Ana Pérez-Lago, Laura Muñoz, Patricia Catalán, Pilar de Viedma, Darío García Microbiol Spectr Observation Reverse transcription-PCRs (RT-PCRs) targeting SARS-CoV-2 variant of concern (VOC) mutations have been developed to simplify their tracking. We evaluated an assay targeting E484K/N501Y to identify B.1.351/P1. Whole-genome sequencing (WGS) confirmed only 72 (59.02%) of 122 consecutive RT-PCR P.1/B.1.351 candidates. Prescreening RT-PCRs must target a wider set of mutations, updated from WGS data from emerging variants. American Society for Microbiology 2021-10-13 /pmc/articles/PMC8515932/ /pubmed/34643437 http://dx.doi.org/10.1128/Spectrum.00816-21 Text en Copyright © 2021 Losada et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Observation
Losada, Carmen
Rico-Luna, Carla
Otero-Sobrino, Álvaro
Molero-Salinas, Andrea
Buenestado-Serrano, Sergio
Candela, Ana
Pérez-Lago, Laura
Muñoz, Patricia
Catalán, Pilar
de Viedma, Darío García
Shared Mutations in Emerging SARS-CoV-2 Circulating Variants May Lead to Reverse Transcription-PCR (RT-PCR)-Based Misidentification of B.1.351 and P.1 Variants of Concern
title Shared Mutations in Emerging SARS-CoV-2 Circulating Variants May Lead to Reverse Transcription-PCR (RT-PCR)-Based Misidentification of B.1.351 and P.1 Variants of Concern
title_full Shared Mutations in Emerging SARS-CoV-2 Circulating Variants May Lead to Reverse Transcription-PCR (RT-PCR)-Based Misidentification of B.1.351 and P.1 Variants of Concern
title_fullStr Shared Mutations in Emerging SARS-CoV-2 Circulating Variants May Lead to Reverse Transcription-PCR (RT-PCR)-Based Misidentification of B.1.351 and P.1 Variants of Concern
title_full_unstemmed Shared Mutations in Emerging SARS-CoV-2 Circulating Variants May Lead to Reverse Transcription-PCR (RT-PCR)-Based Misidentification of B.1.351 and P.1 Variants of Concern
title_short Shared Mutations in Emerging SARS-CoV-2 Circulating Variants May Lead to Reverse Transcription-PCR (RT-PCR)-Based Misidentification of B.1.351 and P.1 Variants of Concern
title_sort shared mutations in emerging sars-cov-2 circulating variants may lead to reverse transcription-pcr (rt-pcr)-based misidentification of b.1.351 and p.1 variants of concern
topic Observation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515932/
https://www.ncbi.nlm.nih.gov/pubmed/34643437
http://dx.doi.org/10.1128/Spectrum.00816-21
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