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Deficiency in the frequency and function of Tr1 cells in IgAV and the possible role of IL-27

OBJECTIVE: Type 1 regulatory T (Tr1) cells are involved in the pathogenesis of numerous immune-mediated diseases. However, little is known about whether and how Tr1 cells affect the development of IgA vasculitis (IgAV). We aimed to investigate this question in IgAV patients. METHODS: . Tr1 cells in...

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Autores principales: Pan, Lu, Wang, Jinghua, Liu, Jinxiang, Guo, Lishuang, Yang, Sirui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8516516/
https://www.ncbi.nlm.nih.gov/pubmed/33280050
http://dx.doi.org/10.1093/rheumatology/keaa752
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author Pan, Lu
Wang, Jinghua
Liu, Jinxiang
Guo, Lishuang
Yang, Sirui
author_facet Pan, Lu
Wang, Jinghua
Liu, Jinxiang
Guo, Lishuang
Yang, Sirui
author_sort Pan, Lu
collection PubMed
description OBJECTIVE: Type 1 regulatory T (Tr1) cells are involved in the pathogenesis of numerous immune-mediated diseases. However, little is known about whether and how Tr1 cells affect the development of IgA vasculitis (IgAV). We aimed to investigate this question in IgAV patients. METHODS: . Tr1 cells in peripheral blood and kidney tissue of IgAV patients were analysed by multi-parametric flow cytometry and immunofluorescence techniques. An in vitro assay of suppression of T cell proliferation and cytokine release was performed to evaluate the function of Tr1 cells. Real-time PCR and cell stimulation in vitro were used to explore the roles of IL-27 and early growth response gene 2 (EGR2). RESULTS: The frequency of Tr1 cells was decreased in peripheral blood but increased in kidney tissue from IgAV patients. A defective suppressive function of Tr1 cells in IgAV was observed. The frequency of Tr1 cells and the cytokines secreted by them were up-regulated in the presence of recombinant IL-27 in vitro. Moreover, IL-27 also increased the expression of EGR2. Furthermore, lower frequency of Tr1 cells during remission had a higher recurrence rate. CONCLUSION: Tr1 cells are involved in the pathogenesis of IgAV. The low IL-27 in IgAV is responsible for impaired frequency and function of Tr1 cells, and EGR2 may be the specific transcription factor involved in the progression. Tr1 may be a risk factor for IgAV recurrence.
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spelling pubmed-85165162021-10-15 Deficiency in the frequency and function of Tr1 cells in IgAV and the possible role of IL-27 Pan, Lu Wang, Jinghua Liu, Jinxiang Guo, Lishuang Yang, Sirui Rheumatology (Oxford) Clinical Science OBJECTIVE: Type 1 regulatory T (Tr1) cells are involved in the pathogenesis of numerous immune-mediated diseases. However, little is known about whether and how Tr1 cells affect the development of IgA vasculitis (IgAV). We aimed to investigate this question in IgAV patients. METHODS: . Tr1 cells in peripheral blood and kidney tissue of IgAV patients were analysed by multi-parametric flow cytometry and immunofluorescence techniques. An in vitro assay of suppression of T cell proliferation and cytokine release was performed to evaluate the function of Tr1 cells. Real-time PCR and cell stimulation in vitro were used to explore the roles of IL-27 and early growth response gene 2 (EGR2). RESULTS: The frequency of Tr1 cells was decreased in peripheral blood but increased in kidney tissue from IgAV patients. A defective suppressive function of Tr1 cells in IgAV was observed. The frequency of Tr1 cells and the cytokines secreted by them were up-regulated in the presence of recombinant IL-27 in vitro. Moreover, IL-27 also increased the expression of EGR2. Furthermore, lower frequency of Tr1 cells during remission had a higher recurrence rate. CONCLUSION: Tr1 cells are involved in the pathogenesis of IgAV. The low IL-27 in IgAV is responsible for impaired frequency and function of Tr1 cells, and EGR2 may be the specific transcription factor involved in the progression. Tr1 may be a risk factor for IgAV recurrence. Oxford University Press 2020-12-06 /pmc/articles/PMC8516516/ /pubmed/33280050 http://dx.doi.org/10.1093/rheumatology/keaa752 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of the British Society for Rheumatology. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Clinical Science
Pan, Lu
Wang, Jinghua
Liu, Jinxiang
Guo, Lishuang
Yang, Sirui
Deficiency in the frequency and function of Tr1 cells in IgAV and the possible role of IL-27
title Deficiency in the frequency and function of Tr1 cells in IgAV and the possible role of IL-27
title_full Deficiency in the frequency and function of Tr1 cells in IgAV and the possible role of IL-27
title_fullStr Deficiency in the frequency and function of Tr1 cells in IgAV and the possible role of IL-27
title_full_unstemmed Deficiency in the frequency and function of Tr1 cells in IgAV and the possible role of IL-27
title_short Deficiency in the frequency and function of Tr1 cells in IgAV and the possible role of IL-27
title_sort deficiency in the frequency and function of tr1 cells in igav and the possible role of il-27
topic Clinical Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8516516/
https://www.ncbi.nlm.nih.gov/pubmed/33280050
http://dx.doi.org/10.1093/rheumatology/keaa752
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