Impact of Bead-Beating Intensity on the Genus- and Species-Level Characterization of the Gut Microbiome Using Amplicon and Complete 16S rRNA Gene Sequencing

Bead-beating within a DNA extraction protocol is critical for complete microbial cell lysis and accurate assessment of the abundance and composition of the microbiome. While the impact of bead-beating on the recovery of OTUs at the phylum and class level have been studied, its influence on species-l...

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Detalles Bibliográficos
Autores principales: Zhang, Bo, Brock, Matthew, Arana, Carlos, Dende, Chaitanya, van Oers, Nicolai Stanislas, Hooper, Lora V., Raj, Prithvi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Cellular and Infection Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517478/
https://www.ncbi.nlm.nih.gov/pubmed/34660333
http://dx.doi.org/10.3389/fcimb.2021.678522

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517478/
https://www.ncbi.nlm.nih.gov/pubmed/34660333
http://dx.doi.org/10.3389/fcimb.2021.678522

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