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Evaluation of an E. coli Cell Extract Prepared by Lysozyme‐Assisted Sonication via Gene Expression, Phage Assembly and Proteomics

Over the past decades, starting from crude cell extracts, a variety of successful preparation protocols and optimized reaction conditions have been established for the production of cell‐free gene expression systems. One of the crucial steps during the preparation of cell extract‐based expression sy...

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Detalles Bibliográficos
Autores principales: Falgenhauer, Elisabeth, von Schönberg, Sophie, Meng, Chen, Mückl, Andrea, Vogele, Kilian, Emslander, Quirin, Ludwig, Christina, Simmel, Friedrich C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8518995/
https://www.ncbi.nlm.nih.gov/pubmed/34240805
http://dx.doi.org/10.1002/cbic.202100257