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Evaluation of an E. coli Cell Extract Prepared by Lysozyme‐Assisted Sonication via Gene Expression, Phage Assembly and Proteomics
Over the past decades, starting from crude cell extracts, a variety of successful preparation protocols and optimized reaction conditions have been established for the production of cell‐free gene expression systems. One of the crucial steps during the preparation of cell extract‐based expression sy...
Autores principales: | Falgenhauer, Elisabeth, von Schönberg, Sophie, Meng, Chen, Mückl, Andrea, Vogele, Kilian, Emslander, Quirin, Ludwig, Christina, Simmel, Friedrich C. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8518995/ https://www.ncbi.nlm.nih.gov/pubmed/34240805 http://dx.doi.org/10.1002/cbic.202100257 |
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