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A gain and dynamic range independent index to quantify spillover spread to aid panel design in flow cytometry

In conventional flowcytometry one detector (primary) is dedicated for one fluorochrome. However, photons usually end up in other detectors too (fluorescence spillover). ‘Compensation’ is a process that corrects the spillover signal from all detectors except the primary detector. Post ‘compensation’,...

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Detalles Bibliográficos
Autores principales: Bhowmick, Debajit, van Diepen, Frank, Pfauth, Anita, Tissier, Renaud, Ratliff, Michelle L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8520008/
https://www.ncbi.nlm.nih.gov/pubmed/34654870
http://dx.doi.org/10.1038/s41598-021-99831-7