Cargando…

Exploitation of E. coli for the production of penicillin G amidase: a tool for the synthesis of semisynthetic β-lactam antibiotics

BACKGROUND: Penicillin G amidase/acylases from microbial sources is a unique enzyme that belongs to the N-terminal nucleophilic hydrolase structural superfamily. It catalyzes the selective hydrolysis of side chain amide/acyl bond of penicillins and cephalosporins whereas the labile amide/acyl bond i...

Descripción completa

Detalles Bibliográficos
Autores principales: Sambyal, Krishika, Singh, Rahul Vikram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8521562/
https://www.ncbi.nlm.nih.gov/pubmed/34652570
http://dx.doi.org/10.1186/s43141-021-00263-7
_version_ 1784584925129736192
author Sambyal, Krishika
Singh, Rahul Vikram
author_facet Sambyal, Krishika
Singh, Rahul Vikram
author_sort Sambyal, Krishika
collection PubMed
description BACKGROUND: Penicillin G amidase/acylases from microbial sources is a unique enzyme that belongs to the N-terminal nucleophilic hydrolase structural superfamily. It catalyzes the selective hydrolysis of side chain amide/acyl bond of penicillins and cephalosporins whereas the labile amide/acyl bond in the β-lactam ring remains intact. MAIN BODY OF ABSTRACT: This review summarizes the production aspects of PGA from various microbial sources at optimized conditions. The minimal yield from wild strains has been extensively improved using varying strain improvement techniques like recombination and mutagenesis; further applied for the subsequent synthesis of 6-aminopenicillanic acid, which is an intermediate molecule for synthesis of a wide range of novel β-lactam antibiotics. Immobilization of PGA has also been attempted to enhance the durability of enzyme for the industrial purposes. SHORT CONCLUSION: The present review provides an emphasis on exploitation of E. coli to enhance the microbial production of PGA. The latest achievements in the production of recombinant enzymes have also been discussed. Besides E. coli, other potent microbial strains with PGA activity must be explored to enhance the yields. GRAPHICAL ABSTRACT: [Image: see text]
format Online
Article
Text
id pubmed-8521562
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-85215622021-10-27 Exploitation of E. coli for the production of penicillin G amidase: a tool for the synthesis of semisynthetic β-lactam antibiotics Sambyal, Krishika Singh, Rahul Vikram J Genet Eng Biotechnol Review BACKGROUND: Penicillin G amidase/acylases from microbial sources is a unique enzyme that belongs to the N-terminal nucleophilic hydrolase structural superfamily. It catalyzes the selective hydrolysis of side chain amide/acyl bond of penicillins and cephalosporins whereas the labile amide/acyl bond in the β-lactam ring remains intact. MAIN BODY OF ABSTRACT: This review summarizes the production aspects of PGA from various microbial sources at optimized conditions. The minimal yield from wild strains has been extensively improved using varying strain improvement techniques like recombination and mutagenesis; further applied for the subsequent synthesis of 6-aminopenicillanic acid, which is an intermediate molecule for synthesis of a wide range of novel β-lactam antibiotics. Immobilization of PGA has also been attempted to enhance the durability of enzyme for the industrial purposes. SHORT CONCLUSION: The present review provides an emphasis on exploitation of E. coli to enhance the microbial production of PGA. The latest achievements in the production of recombinant enzymes have also been discussed. Besides E. coli, other potent microbial strains with PGA activity must be explored to enhance the yields. GRAPHICAL ABSTRACT: [Image: see text] Springer Berlin Heidelberg 2021-10-15 /pmc/articles/PMC8521562/ /pubmed/34652570 http://dx.doi.org/10.1186/s43141-021-00263-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Review
Sambyal, Krishika
Singh, Rahul Vikram
Exploitation of E. coli for the production of penicillin G amidase: a tool for the synthesis of semisynthetic β-lactam antibiotics
title Exploitation of E. coli for the production of penicillin G amidase: a tool for the synthesis of semisynthetic β-lactam antibiotics
title_full Exploitation of E. coli for the production of penicillin G amidase: a tool for the synthesis of semisynthetic β-lactam antibiotics
title_fullStr Exploitation of E. coli for the production of penicillin G amidase: a tool for the synthesis of semisynthetic β-lactam antibiotics
title_full_unstemmed Exploitation of E. coli for the production of penicillin G amidase: a tool for the synthesis of semisynthetic β-lactam antibiotics
title_short Exploitation of E. coli for the production of penicillin G amidase: a tool for the synthesis of semisynthetic β-lactam antibiotics
title_sort exploitation of e. coli for the production of penicillin g amidase: a tool for the synthesis of semisynthetic β-lactam antibiotics
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8521562/
https://www.ncbi.nlm.nih.gov/pubmed/34652570
http://dx.doi.org/10.1186/s43141-021-00263-7
work_keys_str_mv AT sambyalkrishika exploitationofecolifortheproductionofpenicillingamidaseatoolforthesynthesisofsemisyntheticblactamantibiotics
AT singhrahulvikram exploitationofecolifortheproductionofpenicillingamidaseatoolforthesynthesisofsemisyntheticblactamantibiotics